Preventing selection bias in the cell line is a critical aspect of cell line development. Selecting clones based on factors such as stability, antibody secretion, or other metrics can result in a ...
We report that epigenetic silencing causes the loss of function of multi-transcript unit constructs that are integrated using CRISPR-Cas9. Using a modular two color reporter system flanked by ...
Gene knock-out (KO) is a critical method for identifying the functions of coding and non-coding genomic regions. Recent emergence of genome editing tools such as CRISPR/Cas9 can be used to ...