PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification can be ...

A new study shows that saliva tests corrected for cell contamination can accurately track JAK2 mutations in ...

Real time RT–PCR is one of the most widely used laboratory methods for detecting the COVID-19 virus. While many countries ...

Conclusions The overlapping extension PCR method established in this study demonstrates high sensitivity and specificity for amplifying HPV16 in cervical exfoliated cells. This method provides a ...

Researchers have developed a light-induced DNA detection method that enables rapid, PCR-free genetic analysis. Their technique offers ultra-sensitive mutation detection in just five minutes, reducing ...

Currently established rapid methods for sterility testing of advanced therapy medicinal products (ATMPs) are mostly based on growth-based processes or the detection of metabolic activities. However, ...

With the global overuse of antibiotics, the emergence of multidrug-resistant (MDR) bacteria and "superbugs" has become a major threat to human health and food safety. Among these, drug-resistant ...

Watchmaker Genomics launched a PCR-free whole genome sequencing workflow integrating library prep and normalization.

A diagnostic study demonstrated that a multiplex real-time PCR assay achieved an 81 percent pathogen detection rate in pediatric stool samples compared to 43.1 percent for conventional microbiological ...

PCR genetic analysis has been in the spotlight since COVID-19, but light is now further facilitating PCR-free methods. Osaka Metropolitan University scientists have developed a light-induced DNA ...